Antiviral role of grouper STING against iridovirus infection.
作者： Youhua Huang , Zhengliang Ouyang , Wei Wang , Yepin Yu , Pengfei Li
DOI: 10.1016/J.FSI.2015.09.014
关键词:
摘要: Stimulator of interferon genes (STING, also known as MITA, ERIS, MPYS or TMEM173) has been identified a central component in the innate immune response to cytosolic DNA and RNA derived from different pathogens. However, detailed role STING during fish iridovirus infection still remained largely unknown. Here, homolog grouper Epinephelus coioides (EcSTING) was cloned its effects on IFN antiviral activity were investigated. The full-length EcSTING cDNA composed 1590 bp encoded polypeptide 409 amino acids with 80% identity large yellow croaker. Amino acid alignment analysis indicated that contained 4 predicated transmembrane motifs (TMs) N terminal, C-terminal domain (CTD) which consisted dimerization (DD), c-di-GMP-binding (CBD) tail (CTT). Expression profile revealed abundant gill, spleen, brain, skin, liver. Upon stimuli vivo, transcript dramatically up-regulated after challenging Singapore (SGIV), lipopolysaccharide (LPS) polyinosin-polycytidylic (poly I:C). Reporter gene assay showed activated ISRE, zebrafish type I III promoter vitro. Mutant mostly mediated by phosphorylation sites at serine residue S379 S387. Moreover, induced could be impaired overexpression EcIRF3-DN EcIRF7-DN, suggesting IRF3/IRF7 dependent manner. In addition, cytopathic effect (CPE) progression SGIV viral protein synthesis significantly inhibited EcSTING, inhibitory abolished S387 mutant transfected cells. Together, our results demonstrated might an important regulator against infection.
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