Overexpression of malic enzyme isoform 2 in Chlamydomonas reinhardtii PTS42 increases lipid production
作者： Jongrae Kim , Ho Seok Kwak , Sang Jun Sim , EonSeon Jin
DOI: 10.1016/J.BITEB.2019.100239
关键词:
摘要: Abstract Genetic modification of microalgae has been performing to overcome the economic and technical difficulties associated with biodiesel production. In this study, explore a new approach exploiting metabolic pathway for effective lipid production, we engineered Chlamydomonas reinhardtii PTS42 overexpress malic enzyme isoform 2 (ME2) which catalyzes conversion malate pyruvate. Overexpression ME2 resulted in increase mRNA level (around 2–3 fold) pyruvate content (42.4% 44.8% respectively) transgenic M16 M17, respectively. The fatty acid M17 were increased by 23.4 19.9% respectively compared parental lines under nitrogen deprivation condition. However, no significant changes acids composition was observed lines. These results demonstrate that overexpression enhances availability biosynthesis production C. reinhardtii.
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Mariana B. Badia, Cintia L. Arias, Marcos A. Tronconi, Verónica G. Maurino, Carlos S. Andreo, María F. Drincovich, Mariel C.Gerrard Wheeler, Enhanced cytosolic NADP-ME2 activity in A. thaliana affects plant development, stress tolerance and specific diurnal and nocturnal cellular processes. Plant Science. ,vol. 240, pp. 193- 203 ,(2015) , 10.1016/J.PLANTSCI.2015.09.015
Nastassia Shtaida, Inna Khozin-Goldberg, Alexei Solovchenko, Konstantin Chekanov, Shoshana Didi-Cohen, Stefan Leu, Zvi Cohen, Sammy Boussiba, Downregulation of a putative plastid PDC E1α subunit impairs photosynthetic activity and triacylglycerol accumulation in nitrogen-starved photoautotrophic Chlamydomonas reinhardtii Journal of Experimental Botany. ,vol. 65, pp. 6563- 6576 ,(2014) , 10.1093/JXB/ERU374
Simone Zäuner, Wibke Jochum, Tara Bigorowski, Christoph Benning, A Cytochrome b5-Containing Plastid-Located Fatty Acid Desaturase from Chlamydomonas reinhardtii Eukaryotic Cell. ,vol. 11, pp. 856- 863 ,(2012) , 10.1128/EC.00079-12
Frederick Meins, Jr., RNA degradation and models for post-transcriptional gene silencing Plant Molecular Biology. ,vol. 43, pp. 261- 273 ,(2000) , 10.1023/A:1006443731515
E. G. Bligh, W. J. Dyer, A rapid method of total lipid extraction and purification. Biochemistry and Cell Biology. ,vol. 37, pp. 911- 917 ,(1959) , 10.1139/O59-099
M. La Russa, C. Bogen, A. Uhmeyer, A. Doebbe, E. Filippone, O. Kruse, J.H. Mussgnug, Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii. Journal of Biotechnology. ,vol. 162, pp. 13- 20 ,(2012) , 10.1016/J.JBIOTEC.2012.04.006
Victoria H. Work, Randor Radakovits, Robert E. Jinkerson, Jonathan E. Meuser, Lee G. Elliott, David J. Vinyard, Lieve M. L. Laurens, G. Charles Dismukes, Matthew C. Posewitz, Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains Eukaryotic Cell. ,vol. 9, pp. 1251- 1261 ,(2010) , 10.1128/EC.00075-10
Javier A. Gimpel, Vitalia Henríquez, Stephen P. Mayfield, In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity Frontiers in Microbiology. ,vol. 6, pp. 1376- 1376 ,(2015) , 10.3389/FMICB.2015.01376
Jin Liu, Danxiang Han, Kangsup Yoon, Qiang Hu, Yantao Li, Characterization of type 2 diacylglycerol acyltransferases in Chlamydomonas reinhardtii reveals their distinct substrate specificities and functions in triacylglycerol biosynthesis Plant Journal. ,vol. 86, pp. 3- 19 ,(2016) , 10.1111/TPJ.13143
Chiranjib Banerjee, Kashyap K. Dubey, Pratyoosh Shukla, Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges. Frontiers in Microbiology. ,vol. 7, pp. 432- 432 ,(2016) , 10.3389/FMICB.2016.00432