Time-dependent recovery of passive neutrophils after large deformation

摘要: Experiments are performed in which a passive human neutrophil is deformed into an elongated "sausage" shape by aspirating it small glass pipette. When expelled from the pipette recovers its natural spherical approximately 1 minute. This recovery process analyzed according to Newtonian, liquid-drop model variational method used simultaneously solve hydrodynamic equations for low Reynolds-number flow and membrane equilibrium with constant tension. The theoretical gives good fit experimental data ratio of cortical tension cytoplasmic viscosity 1.7 x 10(-5) cm/s (0.17 micron/s). However, when cell held only short time period 5 s or less, then expelled, undergoes initial, rapid elastic rebound suggesting that behaves this instance as Maxwell viscoelastic liquid rather than Newtonian