[25] Human kallikrein and prekallikrein

摘要: Publisher Summary Kallikreins are proteases that specifically liberate kinins from plasma a2-globulin substrates known as kininogens. In plasma, kallikrein exists in a precursor form prekallikrein. can be divided into two classes depending on the source which enzymes isolated. This chapter centers human prekallikrein and kallikrein. The enzymic activity of is measured by methods. It also quantifies rate release determined bioassay or radioimmunoassay. Alternatively, ability to hydrolyze synthetic basic amino acid esters assessed. elaborates Kinin-Releasing Assay Bioassay, utilizes smooth muscle contractions elicited bradykinin, released action kininogen. Esterolytic Assay, methanol N-a-toluenesulfonyl-L-arginine methyl ester (TAME) quantitated modification method Siegelman et al. details purification procedure physical properties lists natural inhibitors.