Heat treatment enhances the antimicrobial activity of (+)‐Catechin when combined with copper sulphate

摘要: UNLABELLED The aim of the study was to compare antimicrobial activities freshly made, heat-treated (HT) and 14 day stored (+)-Catechin solutions with (+)-catechin flavanol isomers in presence copper sulphate. activity investigated when combined different ratios Cu(2+) ; 100°C heat treatment; autoclaving; storage against Staphylococcus aureus. -(+)-Catechin complexation, isomer structure-activity relationships, H2 O2 generation were also investigated. Freshly HT, flavanols showed no activity. While -autoclaved -HT(+)-Catechin similar, HT(+)-Catechin more active than either made (generating ) or (-)-Epicatechin (though it generated less 14 day-(+)-Catechin (which had similar controls-although ). When , terms rates activity, lower (-)-Epigallocatechin gallate greater (+)-Catechin. formed acidic complexes as indicated by pH UV-vis measurements although changes did not account for both complexes. complex which could explain its higher SIGNIFICANCE AND IMPACT OF THE STUDY Natural products attract considerable attention search novel antimicrobials, prebiotics antioxidants. Enhanced biological natural has been demonstrated chemical treatment. This article extends few publications on treatments plant combinations adjuncts, raise pathogens such We that treatment increase (+)-Catechin, a weak found commonly plants Heat readily available resources merits consideration development potent substances use clinical settings agriculture.