Characterization and evolution of the expressed rat ferritin light subunit gene and its pseudogene family. Conservation of sequences within noncoding regions of ferritin genes.

摘要: The iron storage protein ferritin consists of two types subunits different molecular weight, heavy (H) and light (L). rat genome contains approximately 20 copies the L-subunit gene, which we have sequenced seven. One is an expressed gene containing three introns located between alpha-helical domains protein. remaining six characteristics processed pseudogenes. Sequence divergence suggest that these pseudogenes arose 3-12 X 10(6) years ago, well within 30 mouse. By using intron probes derived from L-gene, a homologous second copy has been identified in some Fischer rats. Comparison 5'-untranslated region L-gene with published sequences this human L (Santoro, C., Marone, M., Ferrone, Costanzo, F., Colombo, Minganti, Cortese, R., Silengo, L. (1986) Nucleic Acids Res. 14, 2863-2876) H (Costanzo, Staempfli, S., Santoro, Frank, Delius, H., R. 721-735) genes bullfrog cDNA (Didsbury, J. Theil, E. Kaufman, E., Dickey, F. Biol. Chem. 261, 949-955) show strongly conserved 28-base pair sequence, suggesting translational regulatory function. 5' flanking to those areas L- H-genes. implications for control expression are discussed.