Werner Complexes Viewed Anew: Utilizing Cobalt Coordination Chemistry for ‘Traceless’ Stimuli-Responsive Bioconjugation Involving Therapeutic Nanoparticles, Protein PEGylation, and Drug-(Bio)polymer Conjugates

摘要: Alfred Werner received the Nobel Prize in 1913 for his pioneering work developing coordination theory metal complexes. A crucial experiment leading to widespread acceptance of Werner's involved resolution a set chiral cobalt complexes, specifically cis-[Co(en)2(NH3)X]X2 (X˭Cl, Br). The kinetic inertness Co complexes studied was likely major contributor success this endeavor by providing robust system probe ligand geometry. Here, we revisit classic chemistry and show how it can be utilized field targeted drug delivery bioconjugation amine-containing (bio)molecules. proved quite robust, could used crosslink albumin into nanoparticles (20-500 nm diameter) that exhibited exceptional stability under physiological conditions. pursued as “traceless” linker would degrade reducing conditions, which observed experimentally through degradation our cobal...