Development and validation of a stability indicating uplc method fordetermination of Voriconazole in pharmaceutical formulation

摘要: Simple, rapid, sensitive, accurate, robust & rugged stability indicating analytical method for determination of voriconazole in pharmaceutical formulations is developed and validated by using UPLC applied the determining assay tablets (Vfend®), as there no official monograph UPLC. Chromatography was performed with mobile phase containing sodium dihydrogen ortho phosphate acetonitrile ratio 50:50,adjusted to pH 5.50±0.05 dilute NaOH, a flow rate 0.5mL/min, C-18 column UV detection at 254nm.The linearity, accuracy, ruggedness, robustness, precision bench top sample standard solution. Voriconazole were subjected different stress conditions like acid, alkali, peroxide, thermal, water studies checked its specificity, degradation &stability. The very rapid run time 1 min, robust, stable.