Transglutaminase type 2 affects cell migration through post-translational modification of platelet-derived growth factor-BB.

摘要: Migration is a key cellular function with important implications in cell physiology. Impairment of such observed angiogenesis, cancer, central nervous system development, and many other physiological pathological events. Serum considered among the most potent chemotactic stimuli. Transglutaminase 2 (TG2) involved mentioned processes, suggesting hypothesis that TG2 may modulate movement chemotaxis by acting on serum factors. Cell biology biochemistry studies confirmed this hypothesis, showing human contains signals significantly impaired activated TG2. Bioinformatics indicated one factor potential substrate TG2-dependent transamidation platelet-derived growth factor-BB (PDGF-BB). immunometric experiments carried out U87MG glioma line showed recombinant PDGF-BB pre-incubated calcium-activated lost about 70 % its activity antigenicity. These data indicate TG2-transamidating activity, modification play role modulation PDGF’s features. Further, these findings suggest novel point view to study extracellular functions understand how protein signals, as factors cytokines, act space reach their specific targets.