Cuban Brown Propolis Interferes in the Crosstalk between Colorectal Cancer Cells and M2 Macrophages

摘要: Tumor-associated macrophages (TAMs), primarily the M2 phenotype, are involved in progression and metastasis of colorectal cancer (CRC). Cuban brown propolis (Cp) its main component Nemorosone (Nem) displays an antiproliferative effect on different cells, including CRC cell lines. However, whether Cp Nem could exploit cells by targeting their relationship with TAMs remains to be elucidated. In this study, we differentiated human monocytic THP-1 confirmed transition immunofluorescence (IF) staining, qRT-PCR zymography. An MTT assay was performed determine viability HT-29 co-cultured macrophages. Furthermore, migration invasion abilities were determined Transwell assays expression levels epithelial-mesenchymal (EMT) markers analyzed IF staining. We demonstrated that reduced and, accordingly, activity MMP-9 metalloprotein. Moreover, produce soluble factors positively regulate growth, invasion. These M2-mediated effects counteracted treatments, which also played a role regulating EMT E-cadherin vimentin. Taken together, our results indicate contained interferes crosstalk between TAMs,