Monitoring the chromosome 2 intraerythrocytic transcriptome of Plasmodium falciparum using oligonucleotide arrays.
作者： Karine G Le Roch , Sergei Batalov , Elizabeth A Winzeler , Yingyao Zhou
DOI: 10.4269/AJTMH.2002.67.233
关键词:
摘要: To test the feasibility of using short oligonucleotide probes to monitor transcript levels in Plasmodium falciparum, a microarray was manufactured containing 4,167 (25 base single-stranded) derived from predicted coding region P. falciparum chromosome 2. RNA samples three asexual stages (rings, trophozoites, and schizonts) were labeled hybridized arrays. These results reproducible, transcripts detected for 69% 210 genes on In addition, 145 expressed genes, 1/3 appeared be differentially transcribed during cycle. Some regions transcriptionally silent. Results confirmed by Northern blot analysis quantitative reverse transcriptase-polymerase chain reaction. data validate use relatively 25-mers monitoring expression genome that is 82% AT rich.
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