Genetic diversity assessment in Portugal accessions of Olea europaea by RAPD markers
作者： A. I. Cordeiro , J. F. Sanchez-Sevilla , M. C. Alvarez-Tinaut , M. C. Gomez-Jimenez
DOI: 10.1007/S10535-008-0125-1
关键词:
摘要: Eighty seven olive (Olea europaea ssp. sativa L.) cultivar accessions from Portugal were characterized by means of randomly amplified polymorphic DNA (RAPD) markers. Of the 11 arbitrary 10-mer primers tested a total 92 bands obtained, representing 87.6 % amplification products. Twenty nine different genotypes clearly discriminated. Differences not found among profiles individuals same cultivar. All could be identified combination three primers: OPR-1, OPK-14 and OPA-1, genotype-specific markers being detected. Genetic relationships estimated unweighted pair-group method with arithmetic averaging (UPGMA). The genetic analysis results showed gradual distance between various cultivars, making it difficult to identify well-differentiated phylogenetic groups, although two clusters distinguishable 35 similarity, in addition independent branches lower similarity: Galega, Tentilheira Redondal. dendrogram reflect some for most cultivars according use fruit ecological adaptation.
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