Aging and chloride channel regulation in rat fast-twitch muscle fibres.
作者： A. De Luca , D. Tricarico , S. Pierno , D. Conte Camerino
DOI: 10.1007/BF00585945
关键词:
摘要: By the use of pharmacological tools, we tested hypothesis that age-related alterations in regulatory pathways chloride channels might contribute to lowered conductance (GCl) found skeletal muscle aged rats. The restingGCl extensor digitorum longus (EDL) muscles from adult rats either young (3–4 months old) or (29 was measured by means computerized intracellular microelectrode recordings. In EDL 3 4-month-old rats, 4-β-phorbol 12,13-dibutyrate (4-β-PDB), a direct activator protein kinase C (PKC), decreasedGCl concentration-dependent manner. same effect exerted cholera toxin. effects both phorbol ester and toxin were inhibited staurosporine, thus indicating indirect (via G protein) activation PKC accounts for decrease ofGCl. An increase cytosolic Ca2+ ionophore A23187 also significantly 25%. 4-β-PDB 20-fold more potent blockingGCl than younger controls, blockedGCl 40%. On other hand, ineffective. Our findings support fast-twitch regulation is target aging process.
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