Refactoring the Embden–Meyerhof–Parnas Pathway as a Whole of Portable GlucoBricks for Implantation of Glycolytic Modules in Gram-Negative Bacteria

摘要: The Embden–Meyerhof–Parnas (EMP) pathway is generally considered to be the biochemical standard for glucose catabolism. Alas, its native genomic organization and control of gene expression in Escherichia coli are both very intricate, which limits portability EMP other biotechnologically important bacterial hosts that lack route. In this work, genes encoding all enzymes linear route have been individually recruited from genome E. coli K-12, edited silico remove their endogenous regulatory signals, synthesized de novo following a (GlucoBrick) enables grouping form functional modules at user’s will. After verifying activity several glycolytic mutants E. coli, versatility these GlucoBricks was demonstrated quantitative physiology tests assays carried out Pseudomonas putida KT2440 P. aeruginosa PAO1 as heterologous hosts. Specific configurations wer...