Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles.

摘要: Many studies evaluated the short-term in vitro toxicity of nanoparticles (NPs); however, long-term effects are still not adequately understood. Here, we investigated potential toxic biomedical (polyacrylic acid and polyethylenimine coated magnetic NPs) two industrial (SiO2 TiO2) NPs following different exposure protocols on physiologically models that able to differentiate: L6 rat skeletal muscle cell line biomimetic normal porcine urothelial (NPU) cells. We show cells more sensitive NP then NPU Transmission electron microscopy revealed an uptake into but In cells, obtained a dose-dependent reduction viability increased reactive oxygen species (ROS) formation after 24 h. Following continuous exposure, stable TiO2 polyacrylic (PAA) levels nuclear factor Nrf2 mRNA, suggesting oxidative damage-associated response. Furthermore, internalized PAA hindered differentiation propose use as novel approach for assessment relevant found blood and/or can be secreted urine.