Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species
作者： M. Saikia , Y. T. Singh , A. Bhattacharya , S. Mazumdar-Leighton
DOI: 10.1111/J.1365-2583.2010.01048.X
关键词:
摘要: Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression digestive trypsin and chymotrypsins in larvae feeding a primary host, Persea bombycina Kosterm., comparison to Litsea monopetala Roxb. using vitro proteolytic assays, zymogram analyses with proteinase inhibitors, restriction digestion RNA-PCR amplicons quantitative real-time PCR (RT-PCR). Eight novel members serine gene were identified, including an intron-spliced (AaPb4) seven putative (AaPb2, AaPb4, AaPb12, AaLm4, AaLm6, AaLm19 AaLm29). Midgut transcript levels higher fed P. whereas transcripts encoding L. monopetala. Complex, sequence divergent midgut proteinases may reflect ability lepidopteran feed different host plants. Possible implications plant choice physiology A. are discussed.
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