Label-free aptamer-based partial filling technique for enantioseparation and determination of DL-tryptophan with micellar electrokinetic chromatography.
作者： Rong Huang , Wenming Xiong , Daifang Wang , Longhua Guo , Zhenyu Lin
关键词:
摘要: In this study, a simple and reproducible method for enantioseparation determination of dl-tryptophan (DL-Trp) was developed by using partial filling technique in combination with MEKC. The corresponding L-Trp specific DNA aptamer used as chiral selector. Sodium cholate to form the micelles enhance enantiomers. Effects concentration, time, buffer composition, separation voltage on were evaluated. Mg(2+) Na(+) concentration found effectively affect efficiency reproducibility. Under optimal conditions, D- completely enantioseparated less than 9 min. This aptamer-based partial-filling approach has potential be extended other enantiomers after replacement aptamers.
sci-hub.se 参考文章(34)
Sulay Jhaveri, Manjula Rajendran, Andrew D. Ellington, In vitro selection of signaling aptamers Nature Biotechnology. ,vol. 18, pp. 1293- 1297 ,(2001) , 10.1038/82414
Hoang-Anh Ho, Mario Leclerc, Optical sensors based on hybrid aptamer/conjugated polymer complexes Journal of the American Chemical Society. ,vol. 126, pp. 1384- 1387 ,(2004) , 10.1021/JA037289F
Shigeru. Terabe, Koji. Otsuka, Kunimichi. Ichikawa, Akihiro. Tsuchiya, Teiichi. Ando, Electrokinetic separations with micellar solutions and open-tubular capillaries Analytical Chemistry. ,vol. 56, pp. 111- 113 ,(1984) , 10.1021/AC00265A031
Man-Yung Tong, Tharanga Payagala, Sirantha Perera, Frederick M. MacDonnell, Daniel W. Armstrong, Study of a new chiral selector: Sodium arsenyl-(l)-(+) tartrate for capillary electrophoresis Journal of Chromatography A. ,vol. 1217, pp. 1139- 1148 ,(2010) , 10.1016/J.CHROMA.2009.09.003
Shigeru. Terabe, Koji. Otsuka, Teiichi. Ando, Electrokinetic chromatography with micellar solution and open-tubular capillary Analytical Chemistry. ,vol. 57, pp. 834- 841 ,(1985) , 10.1021/AC00281A014
Y.-H. Lee, T.-I. Lin, Capillary electrophoretic determination of amino acids with indirect absorbance detection Journal of Chromatography A. ,vol. 680, pp. 287- 297 ,(1994) , 10.1016/0021-9673(94)80079-0
Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Béatrice Roy, Christian Perigaud, Eric Peyrin, Aptamer-modified micellar electrokinetic chromatography for the enantioseparation of nucleotides. Analytical Chemistry. ,vol. 81, pp. 1169- 1176 ,(2009) , 10.1021/AC802443J
Corinne Ravelet, Rym Boulkedid, Anne Ravel, Catherine Grosset, Annick Villet, Jennifer Fize, Eric Peyrin, A L-RNA aptamer chiral stationary phase for the resolution of target and related compounds. Journal of Chromatography A. ,vol. 1076, pp. 62- 70 ,(2005) , 10.1016/J.CHROMA.2005.03.132
Juewen Liu, Zehui Cao, Yi Lu, Functional Nucleic Acid Sensors Chemical Reviews. ,vol. 109, pp. 1948- 1998 ,(2009) , 10.1021/CR030183I
Roderic O. Cole, Michael J. Sepaniak, W. L. Hinze, Optimization of binaphthyl enantiomer separations by capillary zone electrophoresis using mobile phases containing bile salts and organic solvent Hrc-journal of High Resolution Chromatography. ,vol. 13, pp. 579- 582 ,(1990) , 10.1002/JHRC.1240130814