Effect of hydration upon the fluidity of intercellular membranes of stratum corneum: an EPR study.

摘要: The principal mechanisms controlling the molecular permeability through skin are associated to intercellular membranes of stratum corneum (SC), outermost layer mammalian skin. It is generally accepted that an increase in fluidity these leads a reduction physical barrier exerted by SC with consequent enhancement permeation different compounds. known water diffusion increases content SC. Using spin labeling method we evaluate effect hydration on at three depths alkyl chain. Increase drastic membrane especially region near membrane/water interface; decreases going deeper inside hydrophobic core. Analysis electron paramagnetic resonance (EPR) parameters as function temperature showed rotational motion depth 16th carbon atom chain experienced phase transition 45 and 60 degrees C. These temperatures were not altered changes A between 28 48 C was observed from segmental polar headgroup (up 12th chain) strongly dependent upon Our results give better characterization SC, main parameter involved control compounds