Rapid Detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring blaVIM-2, blaIMP-1 and blaOXA-23 Genes by Using Loop-Mediated Isothermal Amplification Methods

摘要: Background: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) and Acinetobacter baumannii (CRAB) are the leading causes of nosocomial infections. A rapid sensitive test to detect CRPA CRAB is required for appropriate antibiotic treatment. We optimized a loop-mediated isothermal amplification (LAMP) assay presence blaVIM-2, blaIMP-1, blaOXA-23, which critical components carbapenem resistance. Methods: Two sets primers, inner outer were manually designed as previously described. The LAMP buffer was (at 2mM MgSO4) by testing different concentrations MgSO4. optimal reaction temperature incubation time determined using gradient thermocycler. Then, blaOXA-23 reactions evaluated 120 P. 99 A. clinical isolates. Results: Only one strain 100 isolates harbored whereas none them blaVIM-2. These results indicate that acquisition blaVIM-2 or blaIMP-1 may not play major role in resistance Korea. Fifty two strains 75 contained but alleles. Conclusions: Our demonstrate usefulness diagnosis CRAB.