Proflavine Hemisulfate as a Fluorescent Contrast Agent for Point-of-Care Cytology

摘要: Proflavine hemisulfate, an acridine-derived fluorescent dye, can be used as a rapid stain for cytologic examination of biological specimens. fluorescently stains cell nuclei and cytoplasmic structures, owing to its small amphipathic structure ability intercalate DNA. In this manuscript, we demonstrated the use proflavine dye on number specimens, including normal exfoliated oral squamous cells, cultured human carcinoma leukocytes derived from whole blood specimens using custom-built, portable, LED-illuminated fluorescence microscope. No incubation time was needed after suspending cells in 0.01% (w/v) diluted saline. Images stained had clearly visible well granular cytoplasm, while exhibited bright nuclei, highlighted multilobar nature neutrophils. We also utility quantitative analysis digital images which detect significant morphological differences between different types. have well-defined membranes allowed nuclear ratios, image texture extract features.