Repeat Sequences in the Tomato Genome

作者: Maria Luisa Chiusano , Chiara Colantuono

DOI: 10.1007/978-3-662-53389-5_10

关键词:

摘要: The sequencing of the tomato genome revealed that, though moderated size when compared to most Solanaceae and other plant species, it comprises more than 60 % DNA repeats. This is in contrast with initial estimations assessing that total comprised only about 10–22 repetitive sequences. These preliminary hypotheses were probably biased by presence single-copy within portion high sequence divergence repeat content. Though release first version sequences 2012, complete view repeated regions at level still partial, because difficulties due mainly assembling. However, deeper knowledge on content its distribution was consistently supported cytogenetics, molecular markers reassociation kinetics, accompanied advanced approaches such as Fluorescence In Situ Hybridization (FISH) recently Optical Mapping. techniques helped clarify many principal aspects related organization major classes tomato, contributing a consistent overview this essential part genome. main focus chapter describe from effort associated bioinformatics, considering highly moderately We provide general complexity content, presenting categories their organization. Then we bioinformatics for repeats analysis, focusing common investigations large genomic sequences, well collections available support annotations. Details methods employed analyze (assembly v. 2.40) published 2012 will be presented. description what known concerning therefore overviewed highlighting results or confirmations obtained thanks effort. discussion focused occurrence genome, questions specific role evolution these extended genomes, eukaryotes, remain open.

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