Sequencing and annotation of the Ophiostoma ulmi genome

摘要: The ascomycete fungus Ophiostoma ulmi was responsible for the initial pandemic of massively destructive Dutch elm disease in Europe and North America early 1910. has ravaged tree population globally is a major threat to remaining population. O. also associated with valuable biomaterials applications. It recently discovered that proteins from can be used efficient transformation amylose production bioplastics. We have sequenced 31.5 Mb genome O.ulmi using Illumina next generation sequencing. Applying both de novo comparative annotation methods, we predict total 8639 gene models. quality predicted genes validated variety data sources consisting EST data, mRNA-seq orthologs related fungal species. Sequence-based computational methods were identify candidate virulence-related genes. Metabolic pathways reconstructed highlight specific enzymes may play role virulence. This sequence will useful resource further research aimed at understanding molecular mechanisms pathogenicity by ulmi. facilitate identification necessary industrial biotransformation