Altered micro-ribonucleic acid expression profiles of extracellular microvesicles in the seminal plasma of patients with oligoasthenozoospermia

摘要: Objective To determine whether microRNA (miRNA) expression profile is different in extracellular microvesicles collected from seminal plasma of men with oligoasthenozoospermia, to gain further insight into molecular mechanisms underlying male infertility. Design Microarray quantitative real-time polymerase chain reaction validation and Western blot analysis confirmation. Setting University research clinical institutes. Patient(s) A total 24 men, including 12 oligoasthenozoospermic subfertile normozoospermic men. Intervention(s) None. Main Outcome Measure(s) Statistically significant altered miRNA profiles compared fertile Result(s) Extracellular exosomes were isolated seminal plasma by ultracentrifugation. Presence exosome-specific proteins was confirmed blotting. In the microvesicles, we analyzed 1,205 miRNAs microarray identified 36 levels Seven overexpressed 29 underexpressed Using as an independent method, significantly higher miR-765 miR-1275 lower level miR-15a Conclusion(s) We part events genital tract. These may help understand