Interactions of Elongation Factor 2 (EF-2) with Guanine Nucleotides and Ribosomes

作者: Rustem NURTEN , Engin BERMEK

DOI: 10.1111/J.1432-1033.1980.TB05979.X

关键词:

摘要: Interactions of rat liver elongation factor 2 (EF-2) with guanine nucleotides and ribosomes were studied by equilibrium dialysis sedimentation methods. GDP (Kd= 0.5 μM) or GDP-Mg2+ (Kd=1.57 displayed a higher affinity in the formation binary complex EF-2 than GTP 2.68 μM), GTP-Mg2+ 2.77 guanosine 5′-[β,γ-methylene]triphosphate (GuoPP[CH2]P) 24.0 μM). NaIO4-oxidized (oGDP) 38 oxidized/reduced (orGDP) 27 had lower affinites to binding site on those GTP. However, oGDP, oGTP oGuoPP[CH2]P resulted stable product which could be recovered nitrocellulose filter technique sodium dodecyl sulfate/polyacrylamide gel electrophoresis. In presence new (or different conformation site) for GuoPP[CH2]P-Mg2+ 0.26 9.3 became apparent. The thus appeared favor involving triphosphates. Adenosine diphosphate ribosylated (ADP-Rib-EF-2) its turn bind ribosome high even without 0.18 μM). GuoPP[CH2]P increased some extent ADP-Rib-EF-2 ribosomal 0.05

参考文章(31)
J. Modolell, B. Cabrer, D. Vazquez, The Interaction of Elongation Factor G with N-Acetylphenylalanyl Transfer RNA·Ribosme Complexes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 3561- 3565 ,(1973) , 10.1073/PNAS.70.12.3561
E S Maxwell, O Henriksen, E A Robinson, Interaction of guanosine nucleotides with elongation factor 2. I. Equilibrium dialysis studies. Journal of Biological Chemistry. ,vol. 250, pp. 720- 724 ,(1975) , 10.1016/S0021-9258(19)41955-8
E Bermek, Interactions of adenosine diphosphate-ribosylated elongation factor 2 with ribosomes. Journal of Biological Chemistry. ,vol. 251, pp. 6544- 6549 ,(1976) , 10.1016/S0021-9258(17)32981-2
Noriko Inoue-Yokosawa, Chikako Ishikawa, Yoshito Kaziro, The Role of Guanosine Triphosphate in Translocation Reaction Catalyzed by Elongation Factor G Journal of Biological Chemistry. ,vol. 249, pp. 4321- 4323 ,(1974) , 10.1016/S0021-9258(19)42519-2
Sjur OLSNES, Abraham K. ABRAHAM, Elongation‐Factor‐2‐Induced Sensitization of Ribosomes to Modeccin FEBS Journal. ,vol. 93, pp. 447- 452 ,(1979) , 10.1111/J.1432-1033.1979.TB12842.X
James F. Collins, Samuel Raeburn, Elizabeth S. Maxwell, Aminoacyltransferase II from rat liver. II. Some physical and chemical properties of the purified enzyme and its adenosine diphosphate ribose derivative. Journal of Biological Chemistry. ,vol. 246, pp. 1049- 1054 ,(1971) , 10.1016/S0021-9258(18)62429-9