Immunostimulatory probiotic Lactobacillus rhamnosus HN001 and Bifidobacterium lactis HN019 do not induce pathological inflammation in mouse model of experimental autoimmune thyroiditis.

摘要: Abstract The possibility that intestinal microflora contribute to the pathogenesis of autoimmune diseases has raised issues regarding safety probiotic organisms, especially those with immunostimulating properties, in individuals such immune dysfunctions. In this study, effect consumption lactic acid bacteria (LAB) strains Lactobacillus rhamnosus HN001(HN001) and Bifidobacterium lactis HN019 (HN019) on induction progression experimental thyroiditis (EAT) was investigated CBA/CaH (H-2 k ) mice. HN001 or skim milk were fed mice daily (1–1.5×10 8 cfu/mouse/day) for 5 9 weeks. A mild form EAT induced by subcutaneous injection mouse thyroglobulin (MTg) either Freund's adjuvant (complete incomplete, CFA IFA) lipopolysaccharide (LPS). proliferative responses spleen lymphocyte MTg stimulation vitro presence (and degree) mononuclear cell infiltration thyroid gland tissues examined assess development severity EAT. levels serum anti-MTg antibodies (IgG1 IgG2a) weight index determined detect receiving MTg. Results showed weeks after immunization, 16.67–50% developed glands. Probiotic feeding did not induce full-blown There no differences spleenocytes response PMA between received alone LAB strains.