Human Blood-Circulating Basophils Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

摘要: ABSTRACT Cell-associated HIV-1 infection has been proposed to play a pivotal role in the spread of infection. Granulocytes are category white blood cells, comprising mainly basophils, neutrophils, and eosinophils, participate various inflammatory reactions defense against pathogens. Here, we investigated human granulocytes dissemination HIV-1. These cells were found express variety attachment factors (HAFs). Basophils expressed HAFs dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM3)-grabbing nonintegrin (DC-SIGN), DC immunoreceptor (DCIR), heparan sulfate proteoglycan (HSPG), α4β7 integrin mediated most efficient capture on surface. Neutrophils DCIR demonstrated limited efficiency viral capture. Eosinophils but exhibited little or no virus-binding capacity. Intriguingly, following direct contact with CD4 + T viruses harbored surface basophils transferred cells. The between formation infectious synapses appeared necessary for spread. In HIV-1-infected individuals, frequency remained fairly stable over course disease, regardless depletion emergence AIDS-associated opportunistic infections. Collectively, our results provide novel insights into roles granulocytes, particularly dissemination. Thus, strategies designed prevent basophil-mediated transfer may be developed new form therapy. IMPORTANCE that blood-circulating can mediate trans -infection expression factors, such as C-type lectins, etc., facilitates transfer. patients different levels counts remains during disease. Our We suggest direction development anti-HIV