Enhancement of antibiotic-activity through complexation with metal ions - Combined ITC, NMR, enzymatic and biological studies.

摘要: Alternative solutions need to be developed overcome the growing problem of multi-drug resistant bacteria. This study explored possibility creating complexes antibiotics with metal ions, thereby increasing their activity. Analytical techniques such as isothermal titration calorimetry and nuclear magnetic resonance were used examine structure interactions between Cu(II), Ag(I) or Zn(II) β-lactam antibiotics. The metal-β-lactam also tested for antimicrobial activity, by micro-broth dilution disk diffusion methods, showing a synergistic increase in activity drugs, enzymatic inhibition assays confirming β-lactamases responsible resistance. metal-antibiotic complex concept was proven successful drugs enhanced against β-lactamase-producing highest effects observed formed Ag(I).