Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli : effect of membrane energization
作者： K Tani , H Tokuda , S Mizushima
DOI: 10.1016/S0021-9258(17)44908-8
关键词:
摘要: In the absence of delta mu H+, in vitro translocation proOmpA resulted stable accumulation a possible intermediate addition to transiently accumulating one. The was detected on polyacrylamide gel as two proteinase K-resistant bands corresponding molecular weight about 28,000. appearance appreciably enhanced when oxidized with ferricyanide. No mature OmpA appeared. When reduced dithiothreitol used, other hand, did not appear at all. Upon replacement Cys302 Gly, abolished. treated labeled N-[3H]-ethylmaleimide, whereas that ferricyanide not. ferricyanide-treated translocated into membrane vesicles presence H+. thus and processed N-[3H]ethylmaleimide. It is concluded possessing Cys290-Cys302 disulfide bridge can be without cleavage bridge, H+ imposed. bridge-containing ATP-dependent, its conversion form blocked adenosine 5'-(beta, gamma-imino)triphosphate. early late stages reaction require ATP differently.
osti.gov
sci-hub.st 参考文章(41)
K Yamane, S Matsuyama, S Mizushima, Efficient in vitro translocation into Escherichia coli membrane vesicles of a protein carrying an uncleavable signal peptide. Characterization of the translocation process Journal of Biological Chemistry. ,vol. 263, pp. 5368- 5372 ,(1988) , 10.1016/S0021-9258(18)60725-2
D.L. Foster, R.H. Fillingame, Energy-transducing H+-ATPase of Escherichia coli. Purification, reconstitution, and subunit composition. Journal of Biological Chemistry. ,vol. 254, pp. 8230- 8236 ,(1979) , 10.1016/S0021-9258(19)86880-1
K Tani, K Shiozuka, H Tokuda, S Mizushima, In vitro analysis of the process of translocation of OmpA across the Escherichia coli cytoplasmic membrane. A translocation intermediate accumulates transiently in the absence of the proton motive force. Journal of Biological Chemistry. ,vol. 264, pp. 18582- 18588 ,(1989) , 10.1016/S0021-9258(18)51507-6
L L Chen, P C Tai, Roles of H+-ATPase and proton motive force in ATP-dependent protein translocation in vitro. Journal of Bacteriology. ,vol. 167, pp. 389- 392 ,(1986) , 10.1128/JB.167.1.389-392.1986
S Matsuyama, S Mizushima, Energy-dependent in vitro Translocation of a Model Protein into Escherichia Coli Inverted Membrane Vesicles Can Take Place Efficiently in the Complete Absence of the Cytosol Fraction Journal of Biological Chemistry. ,vol. 264, pp. 3583- 3587 ,(1989) , 10.1016/S0021-9258(18)94106-2
H Yamada, H Tokuda, S Mizushima, Proton motive force-dependent and -independent protein translocation revealed by an efficient in vitro assay system of Escherichia coli. Journal of Biological Chemistry. ,vol. 264, pp. 1723- 1728 ,(1989) , 10.1016/S0021-9258(18)94246-8
M Akita, S Sasaki, S Matsuyama, S Mizushima, SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli. Journal of Biological Chemistry. ,vol. 265, pp. 8164- 8169 ,(1990) , 10.1016/S0021-9258(19)39052-0
K. Cunningham, R. Lill, E. Crooke, M. Rice, K. Moore, W. Wickner, D. Oliver, SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA. The EMBO Journal. ,vol. 8, pp. 955- 959 ,(1989) , 10.1002/J.1460-2075.1989.TB03457.X
C A Kumamoto, P M Gannon, Effects of Escherichia coli secB mutations on pre-maltose binding protein conformation and export kinetics. Journal of Biological Chemistry. ,vol. 263, pp. 11554- 11558 ,(1988) , 10.1016/S0021-9258(18)37994-8
Jonathan A. Rothblatt, David I. Meyer, Secretion in yeast: translocation and glycosylation of prepro-α-factor in vitro can occur via an ATP-dependent post-translational mechanism The EMBO Journal. ,vol. 5, pp. 1031- 1036 ,(1986) , 10.1002/J.1460-2075.1986.TB04318.X