Efficient Method for the Concentration Determination of Fmoc Groups Incorporated in the Core-Shell Materials by Fmoc-Glycine.

摘要: In this paper, we described the synthesis procedure of TiO2@SiO2 core-shell modified with 3-(aminopropyl)trimethoxysilane (APTMS). The chemical attachment Fmoc–glycine (Fmoc–Gly–OH) at surface structure was performed to determine amount active amino groups on basis Fmoc group calculation. We characterized nanostructures using various methods: transmission electron microscope (TEM), scanning microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and X-ray photoelectron (XPS) confirm modification effectiveness. ultraviolet-visible (UV-vis) measurement adopted for quantitative determination present by substitution. nanomaterials were functionalized Fmoc–Gly–OH then fluorenylmethyloxycarbonyl (Fmoc) cleaved 20% (v/v) solution piperidine in DMF. This reaction led formation a dibenzofulvene–piperidine adduct enabling estimation free maximum absorption 289 301 nm UV-vis spectroscopy. calculations loading materials different molar coefficient: 5800 6089 dm3 × mol−1 cm−1 λ = both 7800 8021 nm. obtained results indicate that TiO2@SiO2–(CH2)3–NH2 calculated 6 9 µmol/g. Furthermore, all measurements compared used as model sample.