HYPER RECOMBINATION1 of the THO/TREX Complex Plays a Role in Controlling Transcription of the REVERSION-TO-ETHYLENE SENSITIVITY1 Gene in Arabidopsis

摘要: Arabidopsis REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) represses ethylene hormone responses by promoting receptor ETHYLENE RESPONSE1 (ETR1) signaling, which negatively regulates responses. To investigate the regulation of RTE1, we performed a genetic screening for mutations that suppress insensitivity conferred RTE1 overexpression in Arabidopsis. We isolated HYPER RECOMBINATION1 (HPR1), is required overexpressor (RTE1ox) at seedling but not adult stage. HPR1 component THO complex, which, with other proteins, forms TRanscription EXport (TREX) complex. In yeast, Drosophila, and humans, THO/TREX complex involved transcription elongation nucleocytoplasmic RNA export, its role plants to be fully determined. investigated how RTE1ox The hpr1-5 mutation may affect mRNA as revealed vivo hybridization fluorescein-labeled oligo(dT)45 unidentified nucleus. reduced total nuclear transcript levels similar extent, reduction rate was affected cordycepin treatment, prematurely terminates transcription. defect THO-interacting TEX1 protein TREX export factor SAC3B also levels. SERINE-ARGININE-RICH (SR) proteins are splicing, found SR SR33 co-localized speckles, agreed association human splicing machinery. reveal expression during less likely export. Gene signaling suppression mutation, indicates selectivity affecting consequent response. Thus, components appear have specific roles or selected genes.