Contribution of ductal cells to cytokine responses by human pancreatic islets.

摘要: In type 1 diabetes, autoimmune destruction of pancreatic beta-cells has been attributed to cytokines released from infiltrating immunocytes. Exposure isolated islets leads nitric oxide (NO) production, which can damage beta-cells. Because ductal cells are closely associated with human beta-cells, we examined whether they contribute this process. Isolated were cultured for 48 h various cytokines. The combination interleukin-1beta (IL-1beta) plus interferon-gamma (IFN-gamma) increased production 12-fold while stimulating mRNA expression inducible synthase (iNOS). condition, 10-20% positive the cytokeratin-19 duct marker also stained iNOS protein, whereas no found in control preparations. Comparison magnitude and these that suggests >50% total islet might originate cells. It is concluded a potential source infiltrated by cytokine-releasing