Promoter hypermethylation of death-associated protein kinase gene in cholangiocarcinoma.

摘要: Background Death-associated protein kinase (DAPK) is a Ca2+/calmodulin-regulated Ser/Thr which involved in apoptosis. The aberrant methylation of its promoter region CpG islands may be one the important mechanisms carcinogenesis. We studied relationship status and expression DAPK gene with clinical findings cholangiocarcinoma. Methods Target DNA was modified by sodium bisulfite, coverting all unmethylated, but not methylated, cytosines to uracil, subsequently detected methylation-specific PCR. Moreover, mRNA assessed RT-PCR. Results Aberrant 11 (30.6%) 36 tissue specimens cholangiocarcinoma, 2 (5.6%) adjacent normal tissues. expressed tumor tissues hypermethylation promoter. There were no statistical differences extent differentiation invasion, lymph node metastasis or pathologic type between methylated unmethylated Conclusions frequency cholangiocarcinoma high it offer an effective means for earlier auxiliary diagnosis malignancy. probably suppressed methylation, could become resistant apoptosis immunological surveillance. epigenetically affected associated carcinogenesis