Applying dimorphic yeasts as model organisms to study mycelial growth: Part 1. Experimental investigation of the spatio-temporal development of filamentous yeast colonies

摘要: Colony development of the dimorphic yeasts Yarrowia lipolytica and Candida boidinii on solid agar substrates under glucose limitation served as a model system for mycelial higher filamentous fungi. Strong differences were observed in behaviour both yeasts: C. colonies reached final colony extension which was small compared to size growth field. They formed cell-density profiles steeply declined along radius no biomass decay processes could be detected. The stop coincided with depletion from substrate. These findings supported hypothesis that glucose-limited can regarded populations single cells grow according diffusion-limited mechanism. Y. continued extend after primary nutrient resource, glucose, until covered entire field accomplished by utilization biomass.