Characterization of the peripheral blood transcriptome and adaptive evolution of the MHC I and TLR gene families in the wolf (Canis lupus)

摘要: The wolf (Canis lupus) is one of the most widely distributed terrestrial mammals, because it well adapted to various ecological niches and their corresponding pathogen environments. Immunological competence a crucial factor involved in adapting changing environment fighting infection animals. In this study, peripheral blood transcriptome wolves was generated via RNA-seq advance understanding immunome, with special focus on major histocompatibility complex class I (MHC I) toll-like receptor (TLR) gene families, which are recognition defense. transcriptomic libraries eight originating from Tibet Inner Mongolia were sequenced, approximately 383 million reads generated. Using genome-guided assembly strategy, we obtained 123,851 unigenes, mean length 845 bp an N50 1121 bp. On basis BLAST searches against NCBI non-redundant protein database (Nr), total 36,192 (29.22%) unigenes annotated. For functional classification, 24,663 assigned 13,016 Gene Ontology (GO) terms belonging 51 sub-categories three main GO categories. Additionally, 7682 classified into 6 Kyoto Encyclopedia Genes Genomes (KEGG) categories, represented signal transduction immune system, 16,238 functionally 25 Eukaryotic Orthologous Groups (KOG) We observed overall higher ω (d N/d S) value at antigen-binding sites (ABSs) than non-ABS regions as clear evidence intergenic/intragenic recombination events MHC loci. our analysis revealed that carnivorous TLRs dominated by purifying selection, values each TLR locus ranging 0.173 0.527. However, also found significant instances positive selection acted several codons domains linked species-specific differences recognition. This study represents first attempt characterize highlight investigating system. Balancing have contributed historical evolution genes. Moreover, carnivores undergone adaptive background high level detected