Nanog Is Dispensable for the Generation of Induced Pluripotent Stem Cells
作者： Benjamin A. Schwarz , Ori Bar-Nur , José C.R. Silva , Konrad Hochedlinger
DOI: 10.1016/J.CUB.2013.12.050
关键词:
摘要: Summary Cellular reprogramming from somatic cells to induced pluripotent stem (iPSCs) can be achieved through forced expression of the transcription factors Oct4 , Klf4 Sox2 and c-Myc ( OKSM ) [1–4]. These factors, in combination with environmental cues, induce a stable intrinsic pluripotency network that confers indefinite self-renewal capacity on iPSCs. In addition homeodomain-containing factor Nanog is an integral part [5–11]. Although not required for maintenance cells, it has been reported essential establishment both embryonic (ESCs) blastocysts iPSCs [10, 12]. Here we revisit role direct reprogramming. Surprisingly, find dispensable iPSC formation under optimized culture conditions. We further document -deficient are transcriptionally highly similar wild-type support generation teratomas chimeric mice. Lastly, provide evidence presence ascorbic acid media critical overcoming previously observed block knockout cells.
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