Establishment and phenotypic characterization of three new human myeloma cell lines (U-1957, U-1958, and U-1996).

摘要: Three new human myeloma cell lines (U-1957, U-1958, and U-1996) have been established in vitro. The are Epstein-Barr virus (EBV) negative, monoclonal, aneuploid should thus represent malignant populations not EBV-carrying non-neoplastic B lymphoblastoid lines. origin of the is also suggested by their capacity for production monoclonal complete immunoglobulin (Ig) molecules (U-1957 U-1958) or IgG light chains (U-1996) same type as protein vivo. All morphological features plasmablasts-plasma cells but appear to slightly different stages differentiation. Thus, U-1958 has plasma morphology, expresses only PCA-1 OKT-10 no other antigens, secretes 1.5 micrograms/mL IgG/10(6) cells/24 hours. U-1957 morphology Fc receptors LB-1 antigen addition antigens. This line produces minimal amounts IgG, which appears be secreted. U-1996, finally, a kappa chain producer, plasmablast three chromosomally heterogeneous contain several markers with 14q+ abnormality common characteristic abnormality. These continuous culture approximately 3 years instrumental studies various aspects biology myeloma.