Growth of Rickettsia felis in Drosophila melanogaster S2 Cells

摘要: Abstract Rickettsia felis is an obligate, intracellular, Gram-negative bacterium and a member of the transitional group rickettsiae. This has been shown to grow in vitro amphibian, tick, mosquito cell lines. Here, we present data show growth R. strain LSU Drosophila melanogaster S2 cells, embryonic, hemocytic line with phagocytic properties. was isolated from Ixodes scapularis E6 (ISE6) cells used infect grown at 25°C. By 19 days postinfection, were 100% infected as determined by Acridine Orange Diff-Quik staining. A species-specific qPCR assay demonstrate that kinetics associated culture infection involved lag/adaptation phase, followed continued 20 postinfection. Moreover, organisms observed using transmission electron microscopy polyclonal antibody against spotted fever The ability use D...