Genome-Wide Polyadenylation Site Mapping

摘要: Abstract Alternative polyadenylation site usage gives rise to variation in 3′ ends of transcripts diverse organisms ranging from yeast human. Accurate mapping sites is major biological importance, since the length 3′UTR can have a strong influence on transcript stability, localization, and translation. However, reads generated using total mRNA sequencing mostly lack very end transcripts. Here, we present method that allows simultaneous analysis alternative transcriptome dynamics at high throughput. By produced vitro , precision during protocol be controlled. This illustrated here for budding yeast. this applied any natural or artificially polyadenylated RNA.