Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs.

摘要: Few methods for noninvasive assessment of arterial stiffness and endothelial dysfunction in porcine models are available. The aim this study was to evaluate anesthetized Gottingen minipigs. Pulse-wave velocity (PWV) assessed male minipigs (n = 8; age approximately 60 wk) by using applanation tonometry the carotid femoral arteries. In addition, flow-mediated vasodilation (FMD) vascular ultrasonography brachial artery dysfunction. To reproducibility methods, were intravenous infusion ketamine midazolam examined every other day a total 3 trials. Neither examination nor systolic, diastolic, or mean blood pressure statistically influenced PWV FMD. median interexamination coefficient variation 17% 59% Measured values corresponded largely those clinically healthy humans, but FMD lower than expected lean, young animals. Although ketamine-midazolam anesthesia we used has been associated with minor hemodynamic effects vivo, vitro studies suggest that both drugs vasodilatory. Therefore might have response, contributing modest response concurrent high coefficients noted. We conclude PWV—but not FMD—showed acceptable its potential application models.