Monoclonal antibodies to the ORF5 product of porcine reproductive and respiratory syndrome virus define linear neutralizing determinants
作者： B Pirzadeh , S Dea
DOI: 10.1099/0022-1317-78-8-1867
关键词:
摘要: Complementary DNA encoding the ORF5 gene of a Quebec reference isolate (IAF-Klop) porcine reproductive and respiratory syndrome virus (PRRSV) was cloned into prokaryotic expression vectors pGEX-4T pET21a to produce ORF5-glutathione S-transferase ORF5-polyhistidine fusion proteins. Five hybridoma cell lines producing monoclonal antibodies (MAbs) 25 kDa viral envelope glycoprotein (GP5) were obtained from BALB/c mice immunized with affinity chromatography-purified GST-ORF5 protein. The polypeptide specificity these anti-PRRSV MAbs, belonging IgG1 isotype, confirmed by Western immunoblotting assays recombinant native proteins, radioimmunoprecipitation using [35S]methionine-labelled concentrated extracellular virus. All MAbs showed virus-neutralizing (VN) activity, VN titres ranging 1:32 1:128. Two (IAF-1B8 IAF-8A8) reacted similar modified live attenuated vaccine strain ATCC VR-2332, but all five failed react prototype European strain, Lelystad virus, in indirect immunofluorescence tests. results suggest that are directed serotype-specific linear neutralizing epitopes which not affected absence carbohydrate residues.
sci-hub.st 参考文章(25)
Peter G.W. Plagemann, Volker Moennig, Lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Advances in Virus Research. ,vol. 41, pp. 99- 192 ,(1992) , 10.1016/S0065-3527(08)60036-6
E A Nelson, J Christopher-Hennings, T Drew, G Wensvoort, J E Collins, D A Benfield, Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. Journal of Clinical Microbiology. ,vol. 31, pp. 3184- 3189 ,(1993) , 10.1128/JCM.31.12.3184-3189.1993
T. W. Drew, J. J. M. Meulenberg, J. J. Sands, D. J. Paton, Production, characterization and reactivity of monoclonal antibodies to porcine reproductive and respiratory syndrome virus. Journal of General Virology. ,vol. 76, pp. 1361- 1369 ,(1995) , 10.1099/0022-1317-76-6-1361
H. S. Kim, J. Kwang, I. J. Yoon, H. S. Joo, M. L. Frey, Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line Archives of Virology. ,vol. 133, pp. 477- 483 ,(1993) , 10.1007/BF01313785
Udeni B.R. Balasuriya, N.James MacLachlan, Antoine A.F. De Vries, Paul V. Rossitto, Peter J.M. Rottier, Identification of a Neutralization Site in the Major Envelope Glycoprotein (GL) of Equine Arteritis Virus Virology. ,vol. 207, pp. 518- 527 ,(1995) , 10.1006/VIRO.1995.1112
E. D. Chirnside, A. A. F. de Vries, J. A. Mumford, P. J. M. Rottier, Equine arteritis virus-neutralizing antibody in the horse is induced by a determinant on the large envelope glycoprotein GL. Journal of General Virology. ,vol. 76, pp. 1989- 1998 ,(1995) , 10.1099/0022-1317-76-8-1989
JJM Meulenberg, A Petersen-den Besten, None, Identification and characterization of a sixth structural protein of Lelystad virus: the glycoprotein GP2 encoded by ORF2 is incorporated in virus particles. Virology. ,vol. 225, pp. 44- 51 ,(1996) , 10.1006/VIRO.1996.0573
H. D. Loemba, S. Mounir, H. Mardassi, D. Archambault, S. Dea, Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus. Archives of Virology. ,vol. 141, pp. 751- 761 ,(1996) , 10.1007/BF01718333
G. Wensvoort, C. Terpstra, J.M.A. Pol, E.A. ter Laak, M. Bloemraad, E.P. de Kluyver, C. Kragten, L. van Buiten, A. den Besten, F. Wagenaar, J.M. Broekhuijsen, P.L.J.M. Moonen, T. Zetstra, E.A de Boer, H.J. Tibben, M.F. de Jong, P. van ‘t Veld, G.J.R. Greenland, J.A. van Gennep, M.Th. Voets, J.H.M. Verheijden, J. Braamskamp, Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Veterinary Quarterly. ,vol. 13, pp. 121- 130 ,(1991) , 10.1080/01652176.1991.9694296
H. Mardassi, S. Mounir, S. Dea, Molecular analysis of the ORFs 3 to 7 of porcine reproductive and respiratory syndrome virus, Québec reference strain Archives of Virology. ,vol. 140, pp. 1405- 1418 ,(1995) , 10.1007/BF01322667