High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas.

作者: Niles A. Pierce , Lisa M. Hochrein , Heyun Li

DOI: 10.1021/ACSSYNBIO.1C00037

关键词:

摘要: The activity of a conditional guide RNA (cgRNA) is dependent on the presence or absence an trigger, enabling cell-selective regulation CRISPR/Cas function. cgRNAs are programmable at two ...

参考文章(45)
Joseph N. Zadeh, Conrad D. Steenberg, Justin S. Bois, Brian R. Wolfe, Marshall B. Pierce, Asif R. Khan, Robert M. Dirks, Niles A. Pierce, NUPACK: Analysis and design of nucleic acid systems Journal of Computational Chemistry. ,vol. 32, pp. 170- 173 ,(2011) , 10.1002/JCC.21596
Brian R. Wolfe, Niles A. Pierce, Sequence Design for a Test Tube of Interacting Nucleic Acid Strands. ACS Synthetic Biology. ,vol. 4, pp. 1086- 1100 ,(2015) , 10.1021/SB5002196
Prashant Mali, Luhan Yang, Kevin M Esvelt, John Aach, Marc Guell, James E DiCarlo, Julie E Norville, George M Church, RNA-Guided Human Genome Engineering via Cas9 Science. ,vol. 339, pp. 823- 826 ,(2013) , 10.1126/SCIENCE.1232033
Alexandra E Briner, Paul D Donohoue, Ahmed A Gomaa, Kurt Selle, Euan M Slorach, Christopher H Nye, Rachel E Haurwitz, Chase L Beisel, Andrew P May, Rodolphe Barrangou, None, Guide RNA functional modules direct Cas9 activity and orthogonality. Molecular Cell. ,vol. 56, pp. 333- 339 ,(2014) , 10.1016/J.MOLCEL.2014.09.019
Prashant Mali, John Aach, P Benjamin Stranges, Kevin M Esvelt, Mark Moosburner, Sriram Kosuri, Luhan Yang, George M Church, CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nature Biotechnology. ,vol. 31, pp. 833- 838 ,(2013) , 10.1038/NBT.2675
Lior Nissim, Samuel D Perli, Alexandra Fridkin, Pablo Perez-Pinera, Timothy K Lu, None, Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells Molecular Cell. ,vol. 54, pp. 698- 710 ,(2014) , 10.1016/J.MOLCEL.2014.04.022
Brandon J. Aubrey, Gemma L. Kelly, Andrew J. Kueh, Margs S. Brennan, Liam O’Connor, Liz Milla, Stephen Wilcox, Lin Tai, Andreas Strasser, Marco J. Herold, An Inducible Lentiviral Guide RNA Platform Enables the Identification of Tumor-Essential Genes and Tumor-Promoting Mutations In Vivo Cell Reports. ,vol. 10, pp. 1422- 1432 ,(2015) , 10.1016/J.CELREP.2015.02.002
E. G. Chapman, D. A. Costantino, J. L. Rabe, S. L. Moon, J. Wilusz, J. C. Nix, J. S. Kieft, The structural basis of pathogenic subgenomic flavivirus RNA (sfRNA) production Science. ,vol. 344, pp. 307- 310 ,(2014) , 10.1126/SCIENCE.1250897
L. Cong, F. A. Ran, D. Cox, S. Lin, R. Barretto, N. Habib, P. D. Hsu, X. Wu, W. Jiang, L. A. Marraffini, F. Zhang, Multiplex Genome Engineering Using CRISPR/Cas Systems Science. ,vol. 339, pp. 819- 823 ,(2013) , 10.1126/SCIENCE.1231143