Improved evaporative light scattering detection for carbohydrate analysis.
作者： Luis Condezo-Hoyos , Elena Pérez-López , Pilar Rupérez
DOI: 10.1016/J.FOODCHEM.2015.02.039
关键词:
摘要: Abstract Optimization and validation of evaporative light scattering detector (ELSD), aided by response surface methodology (RSM), has been developed for the liquid chromatography analysis a wide molecular weight (MW) range carbohydrates, including polysaccharides oligosaccharides. Optimal experimental parameters ELSD detection were: 88.8 °C evaporator temperature, 77.9 °C nebulizer temperature 1.1 standard litres per minute nitrogen flow rate. detection, used together with high performance size exclusion (HPSEC) gave linear from 250 to 1000 mg L −1 ( R 2 > 0.998), limits quantitation 4.83–11.67 16.11–38.91 mg L , respectively. Relative deviation was lower than 1.8% intra-day inter-day repeatability apple pectin, inulin, verbascose, stachyose raffinose. Recovery ranged 103.7% 118.3% fructo-oligosaccharides, α-galacto-oligosaccharides disaccharides. Optimized validated is proposed high- low-MW carbohydrates sensitivity, precision accuracy.
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sci-hub.se 参考文章(18)
M. Lafosse, B. Herbreteau, Chapter 30 Carbohydrate analysis by LC and SFC using evaporative light scattering detection Journal of chromatography library. ,vol. 66, pp. 1101- 1134 ,(2002) , 10.1016/S0301-4770(02)80055-0
Katherine Downes, Leon A. Terry, A new acetonitrile-free mobile phase method for LC–ELSD quantification of fructooligosaccharides in onion (Allium cepa L.) Talanta. ,vol. 82, pp. 118- 124 ,(2010) , 10.1016/J.TALANTA.2010.04.003
Eva Gómez-Ordóñez, Antonio Jiménez-Escrig, Pilar Rupérez, Molecular weight distribution of polysaccharides from edible seaweeds by high-performance size-exclusion chromatography (HPSEC). Talanta. ,vol. 93, pp. 153- 159 ,(2012) , 10.1016/J.TALANTA.2012.01.067
Zhenmin Yang, Jun Hu, Mingyue Zhao, Isolation and quantitative determination of inulin-type oligosaccharides in roots of Morinda officinalis Carbohydrate Polymers. ,vol. 83, pp. 1997- 2004 ,(2011) , 10.1016/J.CARBPOL.2010.11.006
Joseph Rashan, Raymond Chen, Developing a versatile gradient elution LC/ELSD method for analyzing cellulose derivatives in pharmaceutical formulations Journal of Pharmaceutical and Biomedical Analysis. ,vol. 44, pp. 23- 28 ,(2007) , 10.1016/J.JPBA.2007.01.015
Mehmet Sefik Tunc, Adriaan R.P. van Heiningen, Characterization and molecular weight distribution of carbohydrates isolated from the autohydrolysis extract of mixed southern hardwoods Carbohydrate Polymers. ,vol. 83, pp. 8- 13 ,(2011) , 10.1016/J.CARBPOL.2010.07.015
Poovan Shanmugavelan, Su Yeon Kim, Jung Bong Kim, Heon Woong Kim, Soo Muk Cho, Se Na Kim, So Young Kim, Young Sook Cho, Haeng Ran Kim, Evaluation of sugar content and composition in commonly consumed Korean vegetables, fruits, cereals, seed plants, and leaves by HPLC-ELSD Carbohydrate Research. ,vol. 380, pp. 112- 117 ,(2013) , 10.1016/J.CARRES.2013.06.024
Jinhui Zhou, Yitao Qi, Joan Ritho, Lili Duan, Liming Wu, Qingyun Diao, Yi Li, Jing Zhao, Analysis of maltooligosaccharides in honey samples by ultra-performance liquid chromatography coupled with evaporative light scattering detection Food Research International. ,vol. 56, pp. 260- 265 ,(2014) , 10.1016/J.FOODRES.2014.01.014
Isabel Márquez-Sillero, Soledad Cárdenas, Miguel Valcárcel, Comparison of two evaporative universal detectors for the determination of sugars in food samples by liquid chromatography Microchemical Journal. ,vol. 110, pp. 629- 635 ,(2013) , 10.1016/J.MICROC.2013.07.008
Xuejun Liu, Ning Ai, Haiyan Zhang, Meizhen Lu, Dengxiang Ji, Fengwen Yu, Jianbing Ji, Quantification of glucose, xylose, arabinose, furfural, and HMF in corncob hydrolysate by HPLC-PDA-ELSD. Carbohydrate Research. ,vol. 353, pp. 111- 114 ,(2012) , 10.1016/J.CARRES.2012.03.029