Membrane tension mediated mechanotransduction drives fate choice in embryonic stem cells
作者： Henry De Belly , Philip H. Jones , Ewa K. Paluch , Kevin J. Chalut
DOI: 10.1101/798959
关键词:
摘要: Changes in cell shape and mechanics frequently accompany fate transitions. Yet how affects the regulatory path- ways controlling is poorly understood. To probe interplay between shape, fate, we used embryonic stem (ES) cells, which spread as they undergo early differentiation. We found that this spreading regulated by a beta-catenin mediated decrease plasma membrane tension. Strikingly, preventing tension obstructs differentiation of ES cells. further find blocking inhibits endocytosis FGF signalling components, direct exit from state. The defects observed can be rescued increasing Rab5a-facilitated endocytosis. Thus, show mechanically-triggered increase regulates Our findings are fundamental importance for understanding biochemical signaling, therefore fate.
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