The complete genome sequence of Candidatus Liberibacter americanus, a bacterium associated with Citrus Huanglongbing in Brazil

摘要: We used PFGE followed by CsCl bisbenzymide centrifugation to obtain sufficient DNA for pyrosequencing of the Ca. Liberibacter americanus (Lam) strain “Sao Paulo” genome. The complete circular genomic sequence Lam is 1,195,201 bp, with an average GC content 31.12%, somewhat lower than other Liberibacters. There are 1,056 predicted genes, 1,002 encoding proteins, 9 rRNA genes and 45 tRNAs. overall gene organization structure genome more similar Lso Las. 951 common Lam, Las, 27 found in but not only 8 Las Lso. Many pseudogenes or truncated were among unique all 3 species. As two prophage confirmed SP2 being 39,941 bp SP1 16,398 size; as one that appears replicate excision plasmid carries putative lysogenic conversion specifically peroxidases a Type Vc secreted adhesin. These adhesin both appeared fragmentary degenerated Lso, indicating their potential citrus host range determination. genomes seem be under selective pressure reduce GC% lose unimportant genes. Although have outer membrane, most required biosynthesis lipopolysaccharide, which can trigger ROS production, missing from