A mouse mammary tumor virus promoter element near the transcription initiation site.
作者： J Pierce , B E Fee , M G Toohey , D O Peterson
DOI: 10.1128/JVI.67.1.415-424.1993
关键词:
摘要: Transcription from the promoter of mouse mammary tumor virus is subject to both positive and negative control by cellular factors, proviral elements that mediate a basal level transcription must in some way respond these regulatory signals. Several such elements, including TATA box, region containing three octamer-related sequences, binding site for nuclear factor 1, have been previously defined. Additional mutations allowed fourth element be identified near initiation between +2 +10. Sequence alterations within this affect vivo vitro. Gel electrophoresis mobility shift DNase I footprinting assays define protein, termed site-binding specifically recognizes promoter. Optimal levels require as demonstrated correlation protein affinity transcriptional activity specific inhibition vitro an oligonucleotide capable titrating transcriptionally active fractions.
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