Targeting species D adenoviruses replication to counteract the epidemic keratoconjunctivitis

摘要: Human adenoviruses are non-enveloped DNA viruses causing various infections; their pathogenicity varies dependent on virus species and type. Although acute infections can sometimes take severe courses, they rarely fatal in immune-competent individuals. Adenoviral conjunctivitis epidemic keratoconjunctivitis hyperacute highly contagious of the eye caused by human adenovirus types within D. Currently there is no causal treatment available to counteract these diseases effectively. The E2B region genome encodes for viral polymerase, which required adenoviral replication. Here we propose novel model systems test this key factor, as a putative target development efficient antiviral therapy based RNA interference. Using our cell lines found that different small interfering RNAs mediate significant suppression (up 90%) expression levels polymerase upon transfection. Moreover, permanent short hairpin most effective led significant, more than tenfold reduction replication group D involved ocular infections.