The American cranberry: first insights into the whole genome of a species adapted to bog habitat.

摘要: The American cranberry (Vaccinium macrocarpon Ait.) is one of only three widely-cultivated fruit crops native to North America- the other two are blueberry spp.) and grape (Vitis spp.). In terms taxonomy, cranberries in core Ericales, an order for which genome sequence data currently lacking. addition, produce a host important polyphenolic secondary compounds, some beneficial human health. Whereas next-generation sequencing technology allowing advancement whole-genome sequencing, major obstacle successful assembly from short-read complex diploid (and higher ploidy) organisms heterozygosity. Cranberry has advantage being (2n = 2x = 24) self-fertile. To minimize issue heterozygosity, we sequenced fifth-generation inbred genotype (F ≥ 0.97) derived five generations selfing originating cultivar Ben Lear. size V. been estimated be about 470 Mb. Genomic sequences were assembled into 229,745 scaffolds representing 420 Mbp (N50 = 4,237 bp) with 20X average coverage. number predicted genes was 36,364 represents 17.7% genome. Of genes, 30,090 assigned candidate based on homology. Genes supported by transcriptome totaled 13,170 (36%). Shotgun genome, coverage 20X, allowed efficient gene calling. identified represent useful collection further study biochemical pathways cellular processes use marker development breeding horticultural characteristics, such as disease resistance.