NuSAP is essential for chromatin-induced spindle formation during early embryogenesis

摘要: Mitotic spindle assembly is mediated by two processes: a centrosomal and chromosomal pathway. RanGTP regulates the latter process releasing microtubule-associated proteins from inhibitory complexes. NuSAP, microtubule- DNA-binding protein, target of promotes formation microtubules near chromosomes. However, contribution NuSAP to cell proliferation in vivo unknown. Here, we demonstrate that expression highly correlates with during embryogenesis adult life, making it reliable marker proliferating cells. Additionally, show deficiency mice leads early embryonic lethality. Spindle NuSAP-deficient cells inefficient chromosomes remain dispersed mitotic cytoplasm. As result sustained checkpoint activity, are unable progress through mitosis, eventually leading caspase activation apoptotic death. Together, our findings essential for and, simultaneously, they underscore importance chromatin-induced assembly.