The small GTP-binding protein rac regulates growth factor-induced membrane ruffling.

摘要: The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also induced ruffling, this response prevented dominant inhibitory mutant rac N17rac1. This suggests that endogenous proteins are required for factor-induced ruffling. addition to later both microinjection some formation stress fibers, process requiring rho proteins. Using N17rac1 we have shown these act through stimulate rho-dependent response. We propose essential components signal transduction pathways linking organization polymerized actin.