Genetic organization and regulation of the xylose degradation genes in Streptomyces rubiginosus.

摘要: The xylose isomerase (xylA) and the xylulose kinase (xylB) genes from Streptomyces rubiginosus were isolated, their nucleotide sequences determined. xylA xylB encode proteins of 388 481 amino acids, respectively. These two are transcribed divergently within a 114-nucleotide sequence separating coding regions. Regulation xyl in S. was examined by fusing promoters to Pseudomonas putida catechol dioxygenase gene integrating fusions into minicircle integration site on chromosome. expression then measured under variety conditions. results indicated that transcription induced D-xylose repressed glucose. Data quantitative S1 mapping consistent with this conclusion suggested had one initiation sites. 40 bp upstream region. sites 20 41 5' its translation codon. Under control appropriate regulatory elements, cloned capable complementing either Escherichia coli isomerase- or kinase-deficient strains. deduced acid protein is highly homologous other microbial isomerases.